The application of single cell gel electrophoresis or Comet assay to human monitoring studies.
نویسندگان
چکیده
OBJECTIVE In the search of new human genotoxic biomarkers, the single cell gel electrophoresis assay has been proposed as a sensible alternative. MATERIAL AND METHODS This technique detects principally single strand breaks as well as alkali-labile and repair-retarded sites. RESULTS Herein we present our experience using the single cell gel electrophoresis assay in human population studies, both occupationally and environmentally exposed. CONCLUSIONS We discuss the assay feasibility as a genotoxic biomarker.
منابع مشابه
Radioresponse of human lymphocytes pretreated with boron and gadolinium as assessed by the comet assay
Background: Boron and gadolinium are among the nuclides that hold a unique property of being a neutron capture therapy agent. Neutron beams have often a considerable portion of gamma rays with fast neutrons. Gamma rays, as beam contaminants, can cause considerable damage to normal tissues even if such tissues do contain high boron concentrations. Materials and Methods: The modification of radio...
متن کاملAssessment of DNA damage induced by butachlor on Cyprinuscarpio(L. 1758) using Single Cell Gel Electrophoresis
Butachlor (N-(butoxymethyl)-2-chloro-N-2, 6-diethylacetanillide) is widely used chloroacetanilide herbicide in Asian region, especially in the north of Iran. Several studies have been reported on toxicity of butachlor, but little information is accessible on DNA damaging potential. Thus, the present study was undertaken to investigate of DNA damage by butachlor in Cyprinuscarpio(Pisces: Cyprini...
متن کاملEvaluation of DNA damage in Cyprinus carpio (L. 1758) exposedto malathion using Single Cell Gel Electrophoresis
Malathion (S-(1, 2-dicarboethoxyethyl) O, O-dimethyl phosphorodithioate) is one of the organophosphate pesticides comprehensively used in agriculture fields throughout the world. In spite of widely used, little information are available about DNA damage in aquatic organisms. Therefore, the present study carried out to investigation of DNA damage induced by malathion in Cyprinus carpio (Pisces: ...
متن کاملAPPLICATION OF TWO-DIMENSIONAL ELECTROPHORESIS AND NIH 3T3 CELL TRANSFECTION ASSAY IN THE STUDY OF TUMOR-AS SOCIATED PROTEINS AND GENOMIC DNA TUMOROGENICITY IN MALIGNANT HUMAN ESOPHAGEAL SPECIMENS
Total protein and DNA extracted from histologically diagnosed normal nonmalignant and esophageal tumor tissues were used for analysis of polypeptides pattern by two-dimensional gel electrophoresis and DNA transforming activity in NIH 3T3 cell transfection assay, respectively. In comparison to normal tissues, eight polypeptides underwent down-regulation or disappeared, while seven polypeptid...
متن کاملEvaluation of incubation temperatures on DNA damages of the Caspian trout (Salmo trutta caspius Kessler, 1877) single cells through developmental stages by Comet assay
Caspian trout (Salmo trutta caspius Kessler, 1877) is an endemic migratory subspecies in the Caspian Sea, possessing ecologic-economic and biodiversity greatly value. Among habitat factors, temperature and its possible effects on the Caspian trout genome were considered in this research. During Embryonic developmental stages (fertilization–hatching), genomic alterations of single cells were ass...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Salud publica de Mexico
دوره 41 Suppl 2 شماره
صفحات -
تاریخ انتشار 1999